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DNA Calculator

Convert between ng/uL and molarity using DNA length

From NanoDrop Reading

From Known Concentration

Enter volume to calculate total amounts

Quick Reference

For NanoDrop Users

  • dsDNA: 1 A260 unit is approximately 50 ng/uL
  • Path length usually 1 cm (check your device)
  • Include dilution factor if sample was diluted
  • DNA length is critical for molarity calculations

General Notes

  • Average MW per bp is approximately 650 g/mol (dsDNA)
  • 1 uM = 1000 nM
  • Copy number = moles x 6.022 x 10^23
  • Always specify DNA length for accurate calculations