Protein Calculator
Comprehensive protein analysis and concentration tools
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Quick Reference
Extinction Coefficients
- Trp: 5,500 M^-1 cm^-1
- Tyr: 1,490 M^-1 cm^-1
- Cys-Cys: 125 M^-1 cm^-1
- Typical protein: 20,000-80,000
Concentration Ranges
- Bradford: 0.02-0.2 mg/mL
- BCA: 0.2-2 mg/mL
- Lowry: 0.01-1 mg/mL
- A280: depends on epsilon value
Storage Tips
- >1 mg/mL usually stable
- Add glycerol for freezing
- pH near pI may precipitate
- Check for aggregation at high conc